Oral Presentation AUS-oMicS 2025

Biomarker Profiling in Human Serum using Benchtop NMR Spectroscopy (#55)

Julien Wist 1 2 , Philipp Nitschke 1 , Hartmut Schaefer 3 , Claire Cannet 3 , Oscar Millet 4 , Jeremy Nicholson 1 5
  1. Murdoch University, Murdoch, WA, WESTERN AUSTRALIA, Australia
  2. Chemistry Department, Universidad del Valle, Cali, Colombia
  3. Bruker Biospin GMBH, Ettlingen, Germany
  4. Precision Medicine and Metabolism Laboratory, CIC bioGUNE, Derio, Spain
  5. Institute of Global Health Innovation Faculty of Medicine, Imperial College London, London, UK

NMR Spectroscopy is a major technology for metabolic analyses of biofluids, especially for lipoprotein quantification in e.g. human plasma. Although, NMR spectroscopy can give quick results at low preparation cost per sample for its biomarker panels, it is still held back by the high capital cost, large instrument footprint, and requirement for cryogenic liquids. Low cost, benchtop NMR systems have been on the rise within the last decade but haven't seen much attention in the field of metabolomics. In fact, the trend in metabolomics rather evolved towards even bigger magnets making use of increased spectral dispersion to accurately extract information from complex biological samples. Yet here we want to demonstrate that benchtop NMR can provide key metabolic markers in human plasma within competitive time frames opening the path for widespread application. In addition, we want to show that benchtop NMR systems provide the necessary stability for inter-lab comparisons as a fundamental requirement for large scale applications.