Chickpeas (Cicer arietinum) can fix nitrogen through symbiotic bacteria (rhizobium) in their root nodules, but the impact of added inorganic nitrogen on chickpea growth and seed traits remains unclear. Here, we have grown eight chickpea genotypes under low N (no supplemental N) or high N (4 mMol N /pot/week – enough to inhibit biological nitrogen fixation) conditions. Significant differences were observed for total pod and seed weight (p<0.01), total seed weight (p<0.03), % crude protein (p<0.0001), total seed N per plant (g) (p<0.0001), total seed protein per plant (g) (p<0.0001), total seed carbon per plant (p<0.01) and average protein per seed (p<0.002) between low and high N applications. We detected 2,565 proteins, including 63 differentially abundant proteins (DAPs) (adj p-value<0.05, abs. Log2FC>1) from all the cultivars and the treatments, using a data-independent acquisition (DIA) approach. The significantly higher proteins within the low N and high N groups enriched with gene ontology terms like cation (iron) ion transport and homeostasis and lipid storage; and nutrient reservoir and defence activities, respectively. In addition, the genotype-dependent responses at the proteome level were also observed due to low and high N application. The Weighted Gene Correlation Analysis indicates that the plant green biomass significantly impacts grain protein composition. Collectively, high N can enhance seed storage proteins and influence protein accumulation across genotypes, offering opportunities for selecting high-quality chickpea varieties.