The goal of proteomics is the quantitative characterization of proteins in biological systems and the correlation of changes in protein abundance to mechanisms of disease. Mass spectrometry-based proteomics is capable of characterizing proteins not only qualitatively but quantitatively as well. Data-independent analysis (DIA) is a commonly-used technique towards this end (1,2). Instrumentation and workflow innovations are needed to enable easier, faster, deeper and more sensitive quantitative identifications of proteins and peptides. ZT Scan DIA is a novel technique that employs a continuously scanning quadrupole to isolate precursor ions for fast, sensitive, time-of-flight (TOF) detection (3,4). In this work, ZT Scan DIA system was used for the label-free quantitation (LFQ) of complex lysate digests mixed at different ratios and analyzed at different on-column loadings. Fast microflow chromatographic separation was employed, using varying active gradient lengths. The resulting data was analyzed using DIANN software. The results show improvements in the precision and accuracy for LFQ using ZT Scan DIA compared to traditional discrete-window DIA methods. Collectively, these results highlight the effectiveness of ZT Scan DIA (using easy-to-set-up methods) for highly accurate quantitation of proteins, providing higher confidence to researchers for disease biomarker discovery.