This poster describes the identification of N-linked glycopeptides using nanoflow liquid chromatography (LC) separation with electron activated dissociation (EAD) fragmentation on the ZenoTOF 7600 system. >1,000 N-glycosylated peptides were identified in glycopeptide-enriched samples from human plasma digests with an EAD-based data-dependent acquisition (DDA) approach. EAD on the ZenoTOF 7600 system is a tunable electron capture-based fragmentation technique that produces unique peptide fragment ions, allowing for the unambiguous assignment of the types and sites of glycosylation post-translational modifications. Data processing using a combination of PEAKS GlycanFinder software and SCIEX OS software enables robust qualitative analysis of the rich spectra generated by the ZenoTOF 7600 System.