Poster Presentation AUS-oMicS 2025

Selective Mapping of Lipids in the Brain Using Desorption Electrospray Ionization Coupled With Multiple Reaction Monitoring Tandem Mass Spectrometry (121113)

Monica Alves 1 , Marios Georgiadis 2 , Michael Zeineh 2 , Inma Cobos 3 , Jeff Nirschl 3 , Vivian Lu 4 , Ryan Leib 4 , Allis Chien 4 , Andrew Baker 5
  1. Waters Australia, Rydalmere, NSW, Australia
  2. Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA
  3. Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
  4. Mass Spectrometry Center, Stanford University, Stanford, CA, USA
  5. Waters Corporation, Pleasanton, CA, USA

Lipid classes such as phosphatidylcholines, ethanolamine plasmalogens and a number of sphingosine-based lipids (ceramides, cerebrosides, and sphingomyelin) have been reported to be dysregulated in the Alzheimer’s brain. Desorption Electrospray Ionization (DESI) as an imaging mass spectrometry source can be used to profile the spatial distribution of lipids and other analytes directly from the surface of a biological sample with minimal sample preparation, thus maintaining the integrity of the analyte distribution.