Poster Presentation AUS-oMicS 2025

Employing Ballistic Gradients, Vacuum Jacketed Columns and a Novel MRT to Increase Lipidomic Throughput Whilst Maintaining Highly Confident Identifications (121080)

Tyren M Dodgen 1 , Scarlet A Ferrinho 2 3 , Preeti Mourya 2 , Shazneil Briones 2 , Nyasha Munjoma 3 , Richard Lock 3 , Robert S Plumb 3 , David Heywood 3 , Lee A Gethings 3 , Olivier Cexus 2 , Paul A Townsend 2
  1. Waters Australia, Waters Corporation, Rydalmere, NSW, Australia
  2. University of Surrey, Surrey, United Kingdom
  3. Waters Corporation, Wilmslow, United Kingdom

One common theme between all omics applications is the ever-increasing size of patient cohorts, driven by the need for identification of novel disease biomarkers and increasing the power of the studies. However, as these studies scale to thousands of patient samples throughput becomes the limiting factor. It may be possible to decrease separation time, however this comes at the cost of peak capacity and feature detection. Vacuum jacketed columns (VJC) can significantly increase peak capacity and narrow peak widths, thereby allowing faster chromatography when combined with fast scanning acquisitions without information loss, or maintaining chromatography duration while increasing peak capacity.