Thermo Scientific TMTpro reagents enable researchers to simultaneously identify and
quantify proteins and peptides from many samples in a single LC-MS/MS experiment.
Current TMTpro isobaric mass tags incorporate 13C & 15N stable isotopes to allow
quantitative analysis of up to 18 samples in parallel by high-resolution MS/MS analysis. To
further increase multiplexing capability, we developed an additional isobaric set of 17
isotopologues that incorporate a single 2H isotope on the reporter group to yield distinct
reporter ion masses that differ from the existing set by 3 mDa. In combination with the
traditional reagent set, the deuterated reagents enable multiplexed quantitative analysis of
up to 35 samples on Thermo Scientific Orbitrap platforms. Here, we characterize the novel
TMTpro variants and assess their performance for 32-plex quantification.